苦丁茶化学成分研究——黄酮类化合物的HPLC分离鉴定

用高效液相色谱法分离鉴定了苦丁茶的黄酮类物质,9个苦丁茶样品分离出12个不同峰,每个样品分别检出6～8个峰.经鉴定,多数苦丁茶样品含有芦丁、杨梅酮和槲皮素,苦丁绿细茶中这3种成分总量在93～851mg/100g之间,为茶叶(绿茶)的1.6～14.7倍;苦丁红细茶加工的发酵过程对这3种成分有破坏作用.苦丁茶黄酮类含量高于茶叶,可能是导致其品质风格与茶叶差异的根本原因.     

苦丁茶复方袋泡茶的研究与开发

以宁波绿茶为基础,添加苦丁茶和茉莉花,进行新颖袋泡茶配方的研制。研究结果表明,配方是6(即A2B3C2)的袋泡茶,在香气和滋味方面都既有耐冲泡和口感平和的特点,又有苦丁茶的甜味,滋味较甘爽,咖啡碱低,是一种优质袋泡茶。     

Arbutin-2'-sulphonyl from the infusion of Ilex theezans leaves

A sulphate arbutin derivative was isolated from the leaves of Ilex theezans, an adulterant of mate (Ilex paraguariensis). The structure was determined by spectral analysis.     

无刺枸骨扦插育苗技术研究

扦插育苗是快速繁育无刺枸骨的有限途径，用高锰酸钾与ABT2号生根粉处理穗条，插穗生根前遮荫，其形成愈伤组织时间比对照提前15d，生根提前14d，成活率提高22％。     

金叶龟甲冬青袋式组培应用效果试验初报

以金叶龟甲冬青组培分化苗为供试材料,采用常规瓶式培养和袋式培养方式进行增殖、生根培养。结果表明：2种培养方式生产的组培苗生长状况无明显差异,均表现出增殖系数大,生长正常,生根快,根系发达等,生根率达92%,且苗木健壮,质量好,移栽成活率高（85%以上）。但在培养基灭菌和接种效率、污染率、移植成活率、培养空间利用率和炼苗效率等方面,袋式培养优于常规瓶式培养,可提高生产效率,达到节能增效目的。     

大叶冬青不同混交造林模式的生长效果比较

采用完全随机区组进行大叶冬青混交试验。结果表明,大叶冬青、马尾松比较适合试验地气候、土壤,马尾松是大叶冬青的较佳混交树种,大叶冬青×马尾松混交能够促进大叶冬青和马尾松的生长,可在同立地条件林地进行推广;建柏、乳源木莲与大叶冬青混交也能不同程度地促进林木的生长,可根据具体情况营造大叶冬青和建柏或乳源木莲的混交林;光皮桦不能适应该立地条件,大叶冬青和光皮桦混交林的生长状况不良。     

金叶日本冬青愈伤组织诱导及分化的研究

以金叶日本冬青新梢茎段为外植体,探讨不同部位新梢、不同激素组合、糖源以及培养基pH值对其愈伤组织诱导及分化的影响.结果表明,采用相同处理,取中上部新梢茎段作外植体较好,其消毒成活率、愈伤萌动率、分化成苗率均较高;MS+KT 1.0 mg/L+NAA 0.2 mg/L培养基对愈伤组织诱导分化最佳;蔗糖浓度为30 g/L时效果最好,愈伤诱导率为100%;培养基pH 值以6.0较适宜.     

Nitrogen fixing activity in warty lenticellate tree barks

Detached lenticellate warty bark of Inga laurina was first shown to develop acetylene reducing activity (ARA) (18 nmol C₂H₂red·g^-1 (F.W.)·hr^-1) after a few days' incubation with ambient air containing 0.10 atm C₂H₂. Similar N₂ase development was found to occur with warty barks of many other plant species including 12 leguminous and 9 non-leguminous trees growing in wet tropical and temperate forests. Among them higher activities (>7 nmol C₂H₂ red·g^-1 (F.W.)·hr^-1) were recorded in Inga laurina, Cynometra ramiflora, Cassia siamea, Robinia pseudo-acacia, Albizia julibrissin in the leguminous plants, and Ilex crenata, Ilex pedunculosa, Rhizophora mucronata, Bruguiera gymnorrhiza and Mallotus japonicus in the non-leguminous plants. The development of ARA was often accelerated under lower pO₂ (0.05 atm) and pC₂H₂ (0.05 atm). Complete replacement of O₂ with N₂ or Ar in the incubation atmosphere resulted in full suppression of the development of N₂ase activity.

When ARA measurement was made with intact barks of natural stands (Robinia pseudo-acacia, Ilex pedunculosa and Mallotus japonicus), it was found that all of these intact barks were reducing C₂H₂ linearly without any time lag. The activities recorded were 4.6–11.5 nmol C₂H₂ red·g^-1 (F.W.)·hr^-1 corresponding to 1.19–1.27 nmol C₂H₂ red·cm^-2hr^-1. These values roughly coincided with ARA of detached warty barks of the same plant species. The present results suggest that in situ N₂-fixation in tree barks of the forests would amount to several 10 kg·ha^-1·year^-1 in temperate and tropical wet forests     

苏州太湖流域2种针阔混交林林分空间结构特征比较

选取太湖流域2种具有代表性的针阔混交林,应用传统林分结构因子配合混交度、角尺度、大小比数和开敞度4个参数进行比较,分析冬青湿地松混交林和栎树湿地松混交林的林分基本特征、空间结构和演替趋势。结果表明,冬青湿地松林的物种丰富度相对较高;对空间结构各参数进行比较,冬青湿地松林空间结构优于栎树湿地松林,林分处于相对稳定状态。其中在冬青湿地松林中,山矾、冬青可能会发展成为优势树种;在栎树混交林中,麻栎和栓皮栎的生长空间受限制,演替过程中优势度会有所下降,短柄粃和构树可能成为优势种。     

不同基质对北美冬青扦插生根的影响

将珍珠岩、泥炭和蛭石按不同比例混合成12种基质,对北美冬青（Ilex verticillata）进行嫩枝扦插试验,研究不同基质对北美冬青扦插生根的影响,结果表明：15%珍珠岩＋35%泥炭＋50%蛭石是北美冬青嫩枝扦插的最佳基质,其生根率为63.89%,平均根长为4.05 cm,根系效果指数达0.65。